Multiplex330TM
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Multiplex 330TM FIELD FLUOROMETER FOR EARLY DISEASE DETECTION Detecting grapevine downy mildew is one of the many applications of the MULTIPLEX 330TM. In the presence of Plasmopara viticola, the downy mildew pathogenic agent, the grapevine leaf produces stilbenes (resveratrol and viniferins) that are fluorescing blue light under UV excitation. Mean spectra of leave (bars = confidence interval) The MULTIPLEX 330TM sensor is a portable fluorometer that allows exciting the fluorescence of molecules under UV, that were unattainable before. The fluorescent molecules, phytoalexins for instance, are measured in real-time and in a non-destructive way. The polyphenols are produced by the plant due to pathogen presence, making the MULTIPLEX 330TM a field fluorimeter for pathogen detection. Abaxial side Excitation spectrum of the blue fluorescence (400 nm) of stilbenes on grapevine leaves infected by downy mildew (adaxial side). The arrow points out the excitation wavelength of the MULTIPLEX 330TM sensor. Adaxial side Inoculated leaves Mean Main indices provided by the MULTIPLEX 330TM sensor: Control leaves Mean ■ BF_UV: blue fluorescence under UV excitation. ■ ANTH: anthocyanin index (stress). ■ FLAV: flavonol index (light, protection). ■ SFR_R and SFR_G: chlorophyll indices (chlorosis). (bars = standard deviation) It is possible to customize the signals measured by the fluorometer, upon request. Days Post Inoculation (DPI) Kinetics of downy mildew development measured by the MULTIPLEX 330 on the leaf adaxial side. On top: fluorescence macroscopy of the abaxial side (UV excitation, detection in the visible spectrum). Sporulation estimated by reflectance imaging ■ Non-destructive and real-time measurements. ■ Geolocated measurements (GPS). ■ Large sampling area (30 cm²). ■ Multispectral (UV-VIS). ■ Datalogger (data classification, SD card). ■ All kinds of plant – leaves and fruits. The MULTIPLEX 330TM sensor is a portable fluorometer for field as well as laboratory measurements having the following features: Sporulation estimated by fluorescence imaging y = 1.48 x + 40 R² = 0.98 Infected area - sporulation (a.u.) Comparison of the BF_UV index of the MULTIPLEX 330TM sensor with surface measurements of leaves covered by sporangiophores o

MULTIPLEX 330TM TECHNICAL SPECIFICATIONS Measured material Any plant material: leaves, needles, berries, fruits and seeds Measured parameters 9 fluorescence signals including BF_UV: blue fluorescence under near UV-B SFR_R and SFR_G: chlorophyll indices FLAV: flavonol index ANTH: anthocyanin index It is possible to customize the signals measured by the fluorimeter, upon request. Measurement process Measured area Acquisition time Storage capacity Measurement mode Data classification Pressure on one of the two main buttons 28 cm² (6 cm diameter), or 12.5 cm² (4 cm diameter) as an option 1...