MINI-PAM-II
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MINI-PAM-II Photosynthesis Yield Analyzer Featuring fully digital leaf clip, touchscreen, and far red LED Setting a New Benchmark for Photosynthesis Research in the Field

MINI-PAM-II Highly portable, robust and compact PAM chlorophyll fluorometer Microsecond timing enables the MINIPAM-II fluorometer to use the same LED as source for PAM measuring light, actinic light and saturation pulses. Measuring light corresponds to µs flashes of constant amplitude, actinic light is quasi-constant light employed to drive photosynthesis, and saturation pulses temporarily saturate primary photosynthesis so that all photosystem II reaction centers are “closed”. Being a PAM fluorometer, the MINI-PAM-II device records only the fluorescence elicited by measuring light....

MINI-PAM-II/B & MINI-PAM-II/R Two fluorometer versions using either blue or red LEDs for measuring and actinic light The color of light emitted by the primary LED distinguishes the BLUE from the RED version of the MINI-PAM-II fluorometer (Fig. 1). The BLUE version (MINI-PAM-II/B) possesses a blue LED emitting maximally around 475 nm which is replaced by a red LED emitting maximally around 655 nm in the RED version (MINI-PAM-II/R). Both versions have a second LED providing far red light for specific excitation of photosystem I. The second difference between the two versions is the spectral...

Accessories Adapting the MINI-PAM-II ANALYZER to the specific properties of photosynthetic samples Light, Temperature and Humidity Sensing Leaf Clip HOLDER 2035-B The leaf clip 2035-B has been devised to record leaf temperature, light intensities at sample level and air humidity. The clip measures photosynthetically active radiation (PAR) by an LS-C Mini Quantum Sensor, a NiCr-Ni thermocouple records leaf temperature, and a capacitive type, temperature-corrected humidity sensor measures relative humidity of air. Calibration factors of PAR and temperature sensors are stored on the internal...

Suspension Cuvette KS-2500 The suspension cuvette includes a 400 μl sample compartment made of stainless steel with PVC exterior. The cuvette is equipped with a 7 mm fiberoptics window adapter, an injection port for Hamilton syringes, and nozzles for connecting an external flow-through water-bath for temperature control. MAgnetic Stirrer with Fiberoptics Holder MKS-2500 The device is equipped with a specially modified stirrer plate to center and hold the KS-2500 Suspension Cuvette. The MKS-2500 Magnetic Stirrer comes with a Perspex base plate with stand bar for mounting fiberoptics on top...

EXAMPLE OF APPLICATION Measuring simultaneously PAM fluorescence, PAR, leaf temperature and relative humidity The MINI-PAM-II combined with the newly developed digital leaf clip 2035-B measures simultaneously PAM fluorescence, photosynthetically active radiation (PAR), leaf temperature and air humidity. The present experiment demonstrates how these parameters vary during a partly cloudy spring day. The photosynthetic sample was an attached leaf of Ilex aquifolia. Figure 1 Figure 1 shows that, during the first half of the day, PAR increased occasionally to values around 500 μmol m-2 s-1 and...

WinControl-3 Software A single, programmable software for light source control, data acquisition and data processing The MINI-PAM-II can be operated by Windows computers running the WinControl-3 software. The same software operates the fluorometers DIVING-PAM, JUNIOR-PAM, and MONITORING-PAM as well as the Universal Light Meter ULM-500. Saturation pulse analysis and automated experimental protocols can be performed under control of the software WinControl-3 but also by the MINI-PAM-II internal software which is active when the MINI-PAMII is operated autonomously. In the latter case,...

O OPTOELECTRONIC UNIT MINI-PAM-II/B (BLUE VERSION) Measuring light: Blue (470 nm) LED, standard modulation frequencies 5 to 25 Hz adjustable in increments of 5 Hz and 100 Hz, measuring light PAR at standard settings = 0.05 |jmol m-2 s-1. Fluorescence at wavelengths greater than 630 nm is measured Actinic light: Same blue LED as for measuring light, maximum actinic PAR = 3000 jmol m-2 s-1, maximum PAR of saturation pulses = 6000 jmol m-2 s-1 adjustable at increments of 500 jmol m-2 s-1 O distance clip 60° 2010-A Design: Metal clip with fiber holder and 11 mm sample hole: 5.5 cm x 1.4 cm (L x...

O LEAF-CLiP HOLDER 2035-B Design: Consisting of a port to position the MINI-PAM/F Fiberoptics and a clip to hold the sample. A circular 1 cm diameter hole of the upper clip part defines the measuring area. Standard distance between fiberoptics tip and measuring area is 8 mm. The port aligns the fiberoptics at an angle of 60° relative to the measuring plane. A mini quantum sensor is positioned on the sample level by a movable Perspex arm, a leaf temperature sensor is mounted below the sample within an up and down movable Plexiglas tube, and a humidity sensor is mounted at 3 cm distance from...

High Quality Instrumentation for Plant Sciences 2.165/10.2014 Subject to change without prior notice Printed in Germany Heinz Walz GmbH Eichenring 6 91090 Effeltrich Germany