Feline Leukemia Virus Antigen/Immunodeficiency Virus Antibody Duo Principle Feline litative detection of the antibody against feline leukemia virus (FeLV) and immunodeficiency virus (FIV) in feline blood. The nitrocellulose membrane of the kit is immobilized with FeLV-specific antigen in test line and with anti-mouse antibody in control line. And also, FeLV-specific antibody is conjugated to the colloidal gold particles. This conjugate is placed on a polyester or glass fiber as conjugate pad. Another membrane of the kit is immobilized with FIV-specific antigen in test line and with anti-mouse antibody in control line. And also, FIV-specific antigen is conjugated to the colloidal gold particles. This conjugate is placed on a polyester or glass fiber as conjugate pad. When the sample is dropped into the sample well on the device the solubilized conjugate migrates with the sample by passive diffusion and both the conjugate and sample come into contact with the antibody that immobilized onto the nitrocellulose. If the sample contains FeLV antigen or FIV antibody, the result is visible as red line within ~10 minutes in the test line on the each membrane. The solution continues to migrate to encounter a control reagent that binds a control conjugate, thereby producing another red control line Materials Provided Feline FeLV Ag/FIV Ab Duo (Art.No. 40401-09/10) contains the following components: 1. 5/10 test devices individually foil-pouched with a desiccant. 2. One (1) assay solution in bottle. 3. 5/10 capillary tubes (having indicator line for 10 µl of specimen). 4. 5/10 anticoagulant tubes. 5. One (1) instruction manual for use. The presence of humidity may decrease the stability of the reagents. Do not use the kit after the expiration date and do not freeze. For veterinary use only. Wear protective gloves while handling samples and wash hands thoroughly after test. 5. Dispose all the specimens and kits properly after test, in accordance with GLP. 6. Never use reagents from another kit. Discard the Assay solution if it is contaminated with bacteria or mold. Storage & Expiration 1. Feline FeLV Ag/FIV Ab Duo should be stored between 2 to 30℃ (36~86℉). 2. Expiration date of this kit is 18 months after its manufacture date. Specimen Collection and Storage 1. Specimen to be tested should be obtained and handled by standard methods for their collections. A. Serum: Allow the blood to clot, then centrifuge to separate the serum. B. Plasma: Collect the whole blood into the tube contained anticoagulants such as EDTA. Centrifuge the blood and separate the plasma. C. Whole blood: Collect an anticoagulated blood sample in EDTA using standard clinical laboratory procedures. Anticoagulated whole blood samples should be tested within 24 hours of drawing. If delays are expected between sampling and testing, the sample should be stored on ice or refrigerated (2~8℃), but should not be frozen. 2. Serum and plasma should be tested as soon as they are prepared. If necessary, they may be stored at 2~8℃ for up to 24 hours or at -20℃ for longer periods. Test Procedure 1. Place all specimens, test devices and Assay solution and allow them to room temperature prior to testing (15~30min). 2. Prepare the test device as you need, and then mark the patients, ID onto the device. Please perform the test immediately after removing the device from foil pouch. 3. Load 1 drop of specimen into the sample well with capillary tubes. The specimen volume to indicator line is 10 µl. 4. Wait for the specimen to absorb (10~20 seconds). 5. Add 3 drops of Assay solution into the sample well. 6. Interpret the testing result within 10 minutes. Do not read the result after 20 minutes. TEST PROCEDURE: (Provides Anticoagulant tube) 4 Specimen absorption (10~20 seconds) 2 Prepare the specimen (Whole blood, plasma or serum) test results Manufactured by Zet Biotech Inc for LifeAssays AB LifeAssays AB, IDEON Science Park, SE-223 70 Lund, Sweden Phone: +46 46 286 54 00, Fax: +46 46 286 54 19, Email: [email protected], Web: www.lif

FeLV Ag/FIV Ab Duo (continued) Interpretation of the Test 1. A color band will appear in the left section of each result windows to show that the test is working properly. This band is the Control band (C). 2. The right section of each result windows indicates the test results. If another color band appears in the right section of the result window, this band is the Test band (T). Anylab FeLV Ag/FIV Ab Duo is designed for primary screening test of antibody against feline immunodeficiency virus. This kit can provide fast and easy way to get a very accurate result, but do not completely exclude...