The Chlorophyll Content Meter for all leafs including very small leaves & difficult to measure samples Applications Applications Chlorophyll content measurement of: Conifer needles Turf grasses Arabidopsis Immature Rice Small leaf grain grasses Fruit CAM plants such as: Agave, Prickly pear cactus & Pineapple Moss & Lichens Stems & petioles The CCM 300 Uses a proven fluorescence ratio technique for chlorophyll content measurement. Features • Direct readout in chlorophyll content Filamentous Algae on rocks • Measurement modes are included for discrete single measurement, and sample averaging of measurements. • Much larger reliable measuring range than absorption style meters. Get better results at higher chlorophyll content Levels. Great for nitrogen management. • Provides reliable results regardless of leaf or sample size, thickness, and shape, with a high degree of correlation with chemical tests. • Almost unlimited measurement storage up to 2 gigabytes of non volatile flash memory. • USB output / files are comma delineated & may be opened directly in Excel or other spread sheets. • Choice of fluorescence ratio readout or direct readout in relative chlorophyll content.

Measures chlorophyll content reliably from 41 mg m-2 to 675 mg m-2 Gitelson 1999 Nitrogen management measurements require comparisons of sample plants to well fertilized plants. However, leaf absorption based technology has been shown to provide reliable measurements up to only about 400 mg m-2. Gitelson’s ratio fluorescence method has been shown to measure reliably up to 675 mg m-2, providing better measurements of well fertilized plants and better results for nitrogen management work. The Chlorophyll Content Meter - for Almost Everything Else Measuring immature rice Measuring Arabidopsis thalia...

How does it work? Without Sample Clip Blue is excitation light Red is mostly fluorescence from PS II at F700 Far red is mostly fluorescence from PS I at F735 Sample Clip Pine needle Leaf cross section Sample clip on single White Pine needle F735 / F700 Fluorescence Ratio Most of the F 700 fluorescence comes form PS II and most of the F735 fluorescence comes from PS I Most of the blue excitation light only penetrates down to the green mesophyll later. For this reason, along with the use of the F700 wavelength, reabsorbed fluorescence is less of problem, and the linear range of measurement is extended...

The graph above shows the fluorescence emission spectra for three different chlorophyll content measurement values. Graphs from Gitelson 1999 (with permission) Total Chlorophyll ing/m2 Chlorophyll measured, ing/m2 The graph on the left shows the F735/F700 ratio measurement vs. total chlorophyll content by chemical test. The center line is a best fit line that shows the linear relationship between the ratio and chemical measurement. The lighter paralell secondary lines represent a single standard deviation.

CCM-300 Chlorophyll Content Meter - for very small leaves and difficult samples The science for measuring chlorophyll content using chlorophyll fluorescence has been well established. The cost for such systems, however, has been much higher than for the more popular light absorption instruments available. As a result, the ability to measure very small samples, curved samples and very thick samples has been out of reach for most budgets. With the CCM 300, Opti-Sciences has engineered a fluorescence solution that is much closer to the cost of absorption techniques. While it is still more cost effective...

References Gitelson A. A., Buschmann C., Lichtenthaler H. K. (1999) “The Chlorophyll Fluorescence Ratio F735/F700 as an Accurate Measure of Chlorophyll Content in Plants” Remote Sens. Enviro. 69:296-302 (1999) Riikonena J., Kettunen N., Gritsevichc M., Hakalac T., Särkkä L.,Tahvonenem R., (2016) Growth and development of Norway spruce and Scots pine seedlings under different light spectra, Environmental and Experimental Botany Volume 121, January 2016, Pages 112–120 Repo T., Launiainen S., Lehto T., Sutinen S., Ruhanen H., Heiskanen J., Laurén A., Silvennoinen R., Vapaavuori E., Finér L. (2016)...