OS30p + Chlorophyll Fluorometer
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The best of both worlds Hand held pulse modulated fluorometer for FV/FM & Fv/F0, along with advanced OJIP protocols Pulse Modulated Tests: FV/FM - A Measure of Maximum Quantum Efficiency FV/FM is the most used parameter for plant stress detection in the world. Research has proven it to be a robust way to measure plant stress that affects photosystem II. The parameter and its protocol also provides the advantage that samples can be measured and compared in the same known dark adapted state. FV/FM has been shown to correlate with carbon assimilation for many types of plant stress. The OS30p+ measuring trace is graphically displayed in color, and F0 is accurately measured using red modulated light. FV/FQ ~ While it does not directly correlate with carbon assimilation, it Is a very sensitive stress detector that is more sensitive than F\//F[j\. It also allows comparison of samples in the same known dark adapted state. Advanced OJIP "JIP" Test - This plant stress testing method provides a high time resolution image of the Kautsky induction curve against a logarithmic time scale. The curves are displayed, and traces can be overlaid and compared on the color instrument screen, or easily recreated from the data file for comparison, to evaluate plant stress. Direct readout of important stress detecting parameters and the overlay of measuring traces, are now immediately possible in the field. The OS30p+ provides a direct read out of the following parameters: OJIP, t100 [is, t300 [is (or K), tFm (or time to FM), A (or area above the curve), M0 (or RC/ABS), PIABS (or performance Index), FD, FM , FV/FM; and Fv/F0, In addition, OptiSciences goes the extra mile, by measuring F0 instead

FV^FM - The most used chlorophyll fluorescence parameter in the world. FV/FM is a normalized parameter that is measured most accurately by a modulated fluorometer. Developed by Kitajima and Butler (1975) and others, it has stood the test of time for measuring maximum quantum efficiency of PSII in plants. It has shown the robust capability to measure plant stresses that affect PSII. In addition, it offers the advantages that it measures plants in a known and repeatable dark adapted state, and that the measurement was designed to correlate with carbon After dark adaptation, modulated...

The “JIP” Test - OJIP OJIP or the “JIP” test is another dark adapted test that has been used for detecting and measuring plant stress. It was discovered by Kautsky (1957) that if the rise in fluorescence, caused by illumination after dark adaptation, was analysed, at high time resolution, there was a distinct curve shape with multiple steps. Using this approach, plant stress that affects PSII can be measured. The latest research shows that O-J is caused by photochemical quenching, J-I is caused by photoelectrochemical quenching, and I-P is associated with the electric trans-thylakoid...

The instrument measuring screen shown on the right allows instant review of the most important information when taking a measurement. • A color graph of the measuring trace is shown using a logarithmic scale of a linear scale • Direct read out of the most used measuring The last 100 measurements, and the important measuring parameters associated with these measurements, can be reviewed and compared as shown on screen to the right. Modulation Si>c The default red light actinic intensity is 3,500 |imols, other intensities are also available. Test Lensrt The graph on the right was made by...

Rugged field instrument designed for hand held use. The OS-30p has been one of most cited research fluorometers in the world in recent years. The system comes with 10 light weight quality, dark adaptation clips that are very affordable,. Pricing allows the purchase of large quantities to fit most budgets. Standard accessories included are a hard shell carrying case, a battery charger, 10 dark adaptation clips, and a USB cable.

Modulated Fluorometer FV/FM , FV/FO Saturation intensity Saturation light source Modulated light source 600- 6000 umols set from 10% to 100% Array of red LEDs 660 nm. red 0.2 to 1.0 :mols. Detection method Pulse modulated Pin photodiode with 700-750 nm band pass filter Test duration 0.1 seconds to 1.5 seconds. The default saturation pulse duration is set at 1.0 second; however, the software takes a rolling eight point 25 ms average to determine FO, and FM, making it idea for both algae and land plants. Modulated light adjustment manual adjustment from 30% to 80% Parameters measured and...

References: Baker N. R., Oxborough K., (2004) Chlorophyll fluorescence as a probe of photosynthetic productivity. From Chapter 3, “Chlorophyll a Fluorescence a Signature of Photosynthesis”, edited by George Papaqeorgiou and Govindjee, published by Springer 2004, PO Box 17, 3300 AA Dordrecht, The Netherlands, pages 66-79 Kautsky H., Hirsch A. (1931) Neuw Versuche zur Kohlensaureassimilation. Naturwissenshaften 19, 964. Kitajima M, Butler WL (1975) Quenching of chlorophyll fluorescence and primary photochemistry in chloroplasts by dibromothymoquinone. Biochim Biophys Acta 376:105-115...