OS5p+
14Pages

Phone 603-883-4400 Fax: 603-883-4410 Email: sales@optisci.com Website: www.optisci.com OS5p+ Pulse Modulated Chlorophyll Fluorometer The Most Advanced Portable System Available — Detection and Measurement of Most Types of Plant Stress New Technology – Designed to allow & measure chloroplast migration qM Stable Internal Actinic Light Source – for reliable field and laboratory quenching tests. Loriaux (2013) FM’ Correction Option or Square Topped Saturation Flash. Wider Range of Programmable Testing Protocols – More than any other portable fluorometer — Rugged Field Portable – Designed for extended field studies and remote locations. 8 Winn Avenue Hu

Better tests allow better science The Most Popular Tests: Y(II): or )F/FM ’ quantum photosynthetic yield of PSII FV/FM: Maximum quantum yield - Photochemical efficiency of PSII FV/FO: A more sensitive stress detector than Fv/Fm. ETR: Electron Transport Rate (with standard PAR clip included) PAR: Photosynthetically Active Radiation value (with standard PAR clip included) T: Leaf temperature (with standard PAR clip included) responsible st migration is NPQ Chloropla t 30% of for up to abou ght levels li at high actinic 3) (Cazzaniga 201 quires rs in nature, re ration, as it occu tense blue...

Unique - Stable, White actinic light source Recently it was found that chloroplast migration was responsible for up to 30% of all NPQ at high actinic light levels. (Cazzaniga 2013), (Dall’Osto 2014) It was also found that chloroplast migration, a high light avoidance mechanism, responded to white light, and to intense blue light, but not significantly to red light. Futhermore, the process takes between 20 to 35 minutes to reach equilibrium or to relax. While the original work regarding fluorescence NPQ and chloroplast migration was done with dicots (Cazzaniga 2013), chloroplast migration in...

Special Setti Equat ions:Klughm R-Phase:On R-Probe:On Chloroplast migration is be responsible for up to about 30% of NPQ at high actinic light levels A stable, internal, white actinic light source with an intense blue spectrum, is used to ensure proper chloroplast migration, as it occurs in nature. Sett ings Equat ions:Kramer Hendrickson lake model protocol Equat ions:Klughm Kramer lake model quenching protocol The OS5p+ includes the widest range of quenching protocols available. Protocols may be used with the standard square top saturation flash, or the Loriaux 2013 FM' correction...

OJIP-The "JIP"Test OJIP or the "JIP" test is another dark adapted test that has been used for detecting and measuring plant stress. It was first described in some detail by Kautsky (1957). Since that time, it has been found that if the rise in fluorescence, caused by illumination after dark adaptation, was analyzed, at high time resolution, there was a distinct curve shape with multiple steps. Using this approach, plant stress that affects PSII can be measured. It has also been shown that some types of plant stress affect specific parts of the OJIP curve. For example, nitrogen stress, at...

Rapid light Curves Y(II) and ETR are designed to be used under steady state photo synthetic lighting conditions. Under variable lighting conditions, Y(II) understates and ETR overstates the real condition. It has been shown by various researchers, that Rapid light Curves provide a more realistic estimate of Rubisco activity under variable light conditions that are found under canopy, and in aquatic environments. For more information contact Opti-Sciences fortheRLC application note. RLC cardinal points & fitted curve Calculation Constants Step Settings Signal Graph: Fitted Data Graph: Eilers...

Innovative PAR Clip - now standard equipment ! Technology Advances The Opti-Science PAR Clip was created to improve upon previous industry designs. By developing a bottom opening PAR Clip, this new model prevents inappropriate opening when measuring leaves above the operators head, or when mounted on a tripod that occurs with some industry designs. As a result, the Opti-Science PAR Clip allows one handed operation, and eliminates two handed operation. This PAR light sensor is positioned to allow measurement of ambient PAR as well as PAR from internal actinic light sources. o Leaf...

FM' correction according to Loriaux 2013 Duration:!..BS Tail:lS Saturation flash shown using the Loriaux 2013 The OS5p+ provides a choice. Either the standard square topped saturation flash or the Loriaux 2013 FM' correction saturation flash may be used. It is available in the Y(II) protocol, quenching protocols, and in the rapid light curve protocol. The window on the upper left hand side says Mode: Multi, for Multiflash. While the saturation flash duration may be changed when using the square topped saturation flash, it is always fixed at 1.0 seconds when using the Loruaux 2013 FM'...

based on Loriaux (2006), & Loriaux, (2013) Saturation pulses used with modulated fluorometers are designed to close all PSII reaction centers. The maximum fluorescence intensity value, of the saturation flash, FM’, is used in most measurements including, quantum yield of PSII MPSII (also called Y(II) or )F /FM’ ), J (or ETR), and in all quenching protocol parameters. While it is possible to reduce or close all reaction centers in a properly dark adapted sample, with a relatively low amount of light, it has been found that in light adapted samples, with a high actinic light history, complete...

Modulation Light Intensity Gain Auto Setup Intensity Flash Width Flash Tail Square Saturation Flash type & width setting Modulation Light Auto Setup Data Managment Multi-Flash - Single Multiple Phased Saturation Flash FM' Correction The FM' correction protocol can also be chosen for use in other protocols such as quenching and light curves. Input of actual values - The average values for leaf absorptance, 0.84, and the ratio of PSII reaction centers to PSI reaction centers, 0.5, are shown as the default values used to determine electron transport rate. The window on the left allows input of...